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. 2017 Feb 23;7:42835. doi: 10.1038/srep42835

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(a) Germination of primary transformed 35 S: AtTOR seeds on PPT (10 mg/L) selection medium. PCR screening of transformants selected on PPT medium with (b) bar gene (550 bp) in T₁ generation. M, λ.EcoRI-HindIII DNA Marker; PC, Positive Control; NC, Negative Control, 1.9 transgenic plants. (c) Southern-blot hybridization analysis of T₂ generation 35 S: AtTOR (Lanes 1–10 represents line numbers TR2.24, TR15.10, TR10.2, TR22.6, TR5.1, TR17.6, TR21.1, respectively) plants were digested with the restriction enzymes AsiSI and ClaI and hybridized with the DIG-dUTP labeled bar gene probe.