FIG 4 .

rDENV1ic-EDI gains binding and neutralization to DENV4 serotype-specific MAb 5H2. (A) Structure of DENV envelope protein dimer showing transplanted residues from DENV4 (orange) and MAb 5H2 epitope contact residues (green). Residues that are part of both the 5H2 MAb epitope and rDENV1ic-EDI virus are shown in orange. rDENV1ic-EDI virus that was recovered contained an additional mutation (light orange) unrelated to the transplanted 5H2 epitope. (B) Viruses were analyzed in an ELISA virus capture assay for their ability to bind DENV4-specific MAb 5H2. (C) Neutralization of viruses in C6/36 focus reduction neutralization test (FRNT) with DENV4-specific MAb 5H2.