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. 2017 Feb 22;2(1):e00380-16. doi: 10.1128/mSphere.00380-16

FIG 5 .

FIG 5 

rDENV1ic-EDI loses binding and neutralization to EDI DENV1 serotype-specific MAbs. (A, D, and G) Structure of DENV envelope protein dimer showing transplanted residues from DENV4 (orange) and MAb epitope contact residues for 1F4 (magenta) (A), 14C10 (purple) (D), and E103 (cyan) (G). Residues that are part of both the MAb epitope and rDENV1ic-EDI virus are shown in orange. rDENV1ic-EDI virus that was recovered contained an additional mutation (light orange) unrelated to the transplanted 5H2 epitope. (B, E, and H) Viruses were analyzed in an ELISA virus capture assay for their ability to bind MAbs 1F4, 14C10, and E103. (C, F, and I) Neutralization of viruses in C6/36 focus reduction neutralization test (FRNT) with MAbs 1F4, 14C10, and E103.