Figure 1. Quantification of IDGF2 protein in Drosophila haemolymph.

(a) Representative western blot image showing 6–24 ng purified recombinant IDGF2 together with haemolymph samples separated on a 10% PAGE. IDGF2 was detected by anti-IDGF2 antibody. (b) Calibration curve derived by plotting the band density of haemolymph IDGF2 against known amounts of recombinant IDGF2 protein (based on three experiments). For haemolymph isolation, wandering L3 larvae were collected, larvae were surface sterilized in 70% ethanol and excess fluid was blotted off on paper towel. 10 larvae were used for isolation. The larvae were opened by gently pulling the epidermis apart with forceps. The haemolymph was collected with a fine glass pipette and immediately frozen. The haemocytes were not removed.