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. 2017 Feb 23;7:43273. doi: 10.1038/srep43273

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The Cl.8+ were grown in different cell culture conditions. CM, complete medium; SFM - supplement-free medium (=medium containing yeast extract and insulin); SFM + IDGF2 - cells in SFM + IDGF2 (16 μg/ml). The growth/survival rates of cells were measured by the direct counting of cells using digital photographs of identical areas (0.8 × 0.8 mm) taken every 24 hrs. Each point represents the mean ± SEM (n = 3). Significant differences (*p < 0.05, **p < 0.01, ***p < 0.001) between SFM and SFM + IDGF2 treatments are indicated by asterisks and were evaluated by Student’s t-test.