Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 23;7:43273. doi: 10.1038/srep43273

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Effect of Ado (15 μM), IDGF2 (16 μg/μl) plus Ado (15 μM), dAdo (30 μM), and IDGF2 (16 μg/μl) plus dAdo (30 μM) on Cl.8+ cell morphology in the top panels and DNA fragmentation (TUNEL staining) in the bottom panels. Green spots represent the nuclei of apoptotic cells. The addition of IDGF2 reduced the number of apoptotic cells in Ado-treated Cl.8+ cells. Bar = 100 μm. (b) BrdU staining (red). The cells were treated with dAdo (30 μM), IDGF2 (16 μg/μl) plus dAdo (30 μM), Ado (20 μM), IDGF2 (16 μg/μl) plus Ado (20 μM). dAdo-treated cells were essentially negative for BrdU incorporation. The addition of IDGF2 to dAdo increased the number of BrdU-positive cells. dAdo treated Cl.8+ cells did not show morphological changes connected with apoptosis, but they did not proliferate. (c) Effects of Ado, dAdo and IDGF2 on the relative proportion of the TUNEL-positive (apoptotic cells) Cl.8+ cells. Concentrations: Ado (15 μM), IDGF2 (16 μg/μl), dAdo (30 μM). (d,e) Effects of Ado, dAdo and IDGF2 on the relative proportion of the BrdU-positive Cl.8+ cells. Concentrations: Ado (20 μM), IDGF2 (16 μg/μl), dAdo (30 μM). Data in the graphs are presented as mean ± SEM (n = 3). Significant differences were evaluated by ANOVA followed by Tukey test and are indicated by different letters (p < 0.05).