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. 2017 Feb 9;2017:5157626. doi: 10.1155/2017/5157626

Figure 6.

Figure 6

Confirmation of RNA-seq data by RT-qPCR during VACV infection. Gene expression for the indicated genes was assessed by RT-qPCR from VACV- or VACVΔB18-infected L929 cells at 4 hpi and 9 hpi. IFN was added to infected cells at 5 hpi where indicated. Expression levels of WR092 and WR127 VACV genes were also determined to monitor the progress of infection and relativized to VACV 4 hpi expression values. Mean from 3 biological replicates ± SEM and significant differences are displayed.