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. Author manuscript; available in PMC: 2017 Jun 5.
Published in final edited form as: Nat Methods. 2016 Dec 5;14(2):195–200. doi: 10.1038/nmeth.4108

Figure 4.

Figure 4

Lineage tracing in cultured cell populations. (A,B) Passaging scheme: cells carrying hgRNA-A21 (panel A) or hgRNA-C21 (panel B) were induced to express briefly Cas9, generating a parent population (‘0’) with a diverse array of hgRNA barcodes. As shown by arrows, the parent population (‘0’) was passaged into multiple first generation subpopulations (0,N), some of which were then further passaged into second and third generation subpopulations (0,N,N and 0,N,N,N respectively). The ovals represent populations and the numbers in the ovals represent the label of that line while indicating its relationship to the other lines. Each daughter subpopulation was seeded from about 100 cells that were briefly induced to express Cas9 immediately before seeding. (C,D) Clustering of subpopulations in panels A and B based on the hgRNA variants observed in each (Supplementary table 2).