Skip to main content
. 2017 Feb 23;7:43455. doi: 10.1038/srep43455

Figure 2. Sub-cellular localization of TVBMV 6K1 and 6K2 in N. benthamiana epidermal cells.

Figure 2

(a) Transient expression of 6K1-GFP or 6K1-DsRed in N. benthamiana leaf epidermal cells. (b) Localization of 6K1-DsRed in a TVBMV-GFP infected cells. Arrows point to 6K1-DsRed punctate bodies. (c) Co-localization of 6K1-DsRed and 6K2-GFP in association with chloroplasts in a TVBMV infected N. benthamiana epidermal cell. Images (left to right) show GFP fluorescence, DsRed fluorescence, chloroplast auto-fluorescence, and overlay of the first three images, respectively. Arrows indicate the co-localized fluorescence signal. Arrowheads point to chloroplasts. (d) Co-localization of 6K1-GFP and 6K2-DsRed adjacent to chloroplasts. Images are arranged as indicated in (c). (e) Images of N. benthamiana cells co-expressing 6K1-YC and 6K2-YN, 6K1-YC and YN, or YC and 6K2-YN. Arrows point to 6K1–6K2 complexes and arrowheads point to chloroplasts. (f) Co-localization of 6K1-GFP and NIb-DsRed in a TVBMV infected N. benthamiana epidermal cell. Arrows point to 6K1-GFP or NIb-DsRed punctates and arrowheads point to chloroplasts. Images are arranged as indicated in (c). All images were taken at 48 hpai. Scale bars = 10 μm.