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. 2017 Feb 23;7:43455. doi: 10.1038/srep43455

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Co-immunoprecipitation (Co-IP) assay for 6K2 and NbPsbO1. 6K2-GFP and NbPsbO1-HA were co-expressed in N. benthamiana leaves via agro-infiltration. N. benthamiana leaves co-expressing 6K1-GFP and NbPsbO1-HA, or GFP and NbPsbO1-HA were used as negative controls. (b) Images of N. benthamiana cells co-expressing 6K2-YN and NbPsbO1-YC, 6K1-YN and NbPsbO1-YC, YN and NbPsbO1-YC, 6K2-YN and YC. Punctates containing 6K2-YN and NbPsbO1-YC were indicated with arrows. (c) Transient expression of NbPsbO1-GFP and NbPsbO1-DsRed in N. benthamiana leaf epidermal cells. Images (left to right) show NbPsbO1-GFP green punctates, overlay of NbPsbO1-GFP green punctates with chloroplast auto-fluorescence, NbPsbO1-DsRed red punctates, and overlay of NbPsbO1-DsRed red punctates with chloroplast auto-fluorescence, respectively. (d) Co-localization of 6K2-DsRed and NbPsbO1-GFP in association with a chloroplast. Images (left to right) show 6K2-DsRed red punctate, NbPsbO1-GFP green punctate, chloroplast auto-fluorescence, and overlay of the three images. (e) Localization of NbPsbO1-DsRed punctate and 6K1-GFP fluorescence in a cell. Images (left to right) show NbPsbO1-DsRed red punctate, 6K1-GFP fluorescence, chloroplast auto-fluorescence, and overlay of the three images. (f) Co-expression of NbPsbO1-DsRed, 6K1-GFP and non-tagged 6K2 in a cell. Images (left to right) show NbPsbO1-DsRed fluorescence, 6K1-GFP fluorescence, chloroplast auto-fluorescence, differential interference contrast and overlay of the four images. All images were taken at 48 hpai. Scale bars in (c), (d and f) equal to 1 μm; Scale bars in others panels equal to 10 μm.