Fig. 5.—

RT-PCR analysis of ultra-conserved elements. M represents DNA size marker, in which Rox2 shows 100–500 bp bands in 100 bp increments plus a 650 bp band; all other samples show the 100 bp band. Lanes 1–10 are RT-PCRs from cDNA synthesized using oligo-dT, lanes 11–20 are RT-PCRs from cDNA synthesized using random hexamers, 21–30 are RT- controls using random hexamer. Lanes 1, 2, 11, 12, 21, and 22 are RNA extracted from 0 to 4 h embryos; 3, 4, 13, 14, 23, and 24 are from 10 to 20 h embryos; 5, 6, 15, 16, 25, and 26 are from 3 - to 4-day-old larva; 7, 8, 17, 18, 27, and 28 are from 3- to 5-day-old female flies; 9, 10, 19, 20, 29, and 30 are from 3- to 5-day-old male flies; lane 31 is a PCR positive control using genomic DNA as template, lane 32 is PCR negative control using water as template.