Fig. 3.

Accumulation of RSV proteins in Mavs^−/− and Ifnar1^−/− primary AMs. Primary AMs from wt, Mavs^−/−, and Ifnar1^−/− mice were exposed to medium or MOI of 2 of RSV or UV-RSV for 21 h before being fixed, stained using polyclonal-anti-RSV antibodies and DAPI, and analysed by fluorescence microscopy. a Representative brightfield images of medium-exposed AMs. Representative images of UV-RSV-exposed primary AMs (b) or RSV-exposed primary AMs (c) where location of ‘inclusion-like bodies’ are presented by white arrows. Scale bar represents 10 μm. Images characterise the appearance of the majority of AMs from at least 4 independent experiments.