Fig. 4.

IBs are only present in AMs deficient in MAVS and IFNAR1 signalling. Primary AMs from wt, Mavs^−/−, and Ifnar1^−/− mice were exposed to MOI of 2 of RSV for 21 h. Cells were fixed and stained using anti-RSV-N (green), anti-LAMP-1 (red), and DAPI (blue), and examined by fluorescence microscopy. Representative image of medium-exposed AMs (a) and UV-RSV-exposed AMs (b). c RSV-exposed AMs where the location of IBs is presented by white arrows. Images represent the appearance of the majority of AMs from 3 independent experiments. The scale bars represent 10 μm. Monochrome images were converted to composite images using the Fiji software program (ImageJ). d Graphs indicate the pixel intensity of RSV-N, Lamp-1, and DAPI along the white line indicated on the micrographs.