Fig. 5.

The presence of RSV nucleocapsid protein is increased in MAVS- and IFNAR1-deficient AMs. Primary wt, Mavs^−/−, and Ifnar1^−/− AMs were exposed to UV-RSV or RSV (MOI of 2) for 21 h. Cell lysate was generated using RIPA buffer and then subjected to Western blot analysis. The blots were exposed to a polyclonal anti-RSV antibody or to an anti-β-actin antibody. The protein standard is indicated to the right of the blot and the detected RSV proteins (G, N, L, and M proteins) are indicated at the right of the blots. The blots are representative of at least 3 experiments.