Fig. 6.

Control of replication of RSV in AMs is independent of MAVS and IFNAR signalling. a AMs from wt, Mavs^−/−, and Ifnar1^−/− mice and LA4 cells were exposed to medium or MOI of 2 of UV-RSV (AM UV or LA4 UV) or RSV for 1, 24, and 48 h. RSV L and N gene copies were determined by RT-PCR and the data are shown as fold increase over the copies present at 1 h after inoculation. The UV AM show pooled measurements of L and N genes from all genotypes of AMs. b Positive- and negative-sense strands of RSV were detected using RT-PCR. The data are shown as means ± SEM of 3-4 individual RNA samples pooled from 3 independent experiments (a) and 4-6 individual RNA samples pooled from 4 independent experiments (b). Each RNA sample was extracted from 2-3 pooled individual cultures of AMs or LA4 cells. Statistical significance of differences between indicated groups was determined by unpaired Student's t test. In a ‘*’ represents differences between LA4 and wt AM, ‘$’ between wt and Ifnar1^−/− AMs and ‘#’ between wt and Mavs^−/− AMs. * p < 0.05; ** p < 0.01; *** p < 0.001.