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a Schematic diagram for construction of recombinant proteins expression vector system. b SDS–PAGE gel stained with Coomassie Brilliant Blue. Each lane was loaded with 2 µg of purified recombinant proteins. Lanes: M, proteins marker; 1, 5BT (18.1 kDa); 2, B5BT (45.8 kDa); 3, BmpB (28.7 kDa); 4, BSA (66.5 kDa). c Western blot analysis recombinant proteins were detected with His-tag using anti-His-tag antibody. BmpB is a positive control and commercial BSA is a negative control
