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. 2017 Feb 22;16:33. doi: 10.1186/s12934-017-0648-2

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — SDS–PAGE analysis for the expression pattern of recombinant proteins. Lanes: M, protein marker; S, soluble crude protein; IB, crude inclusion bodies; 5BT, 18.1 kDa; B5BT, 45.8 kDa; BmpB, 28.7 kDa. The stained gel was analyzed by image J software and inclusion body fractions were: 5BT, 36%; B5BT, <2%; BmpB, <1%