Fig. 5.

Validation of recombinant proteins as FMDV subunit vaccine in vivo. a Schematic view of immunization and blood sapling schedule (n = 5/group). b Antibody titer analysis by ELISA. Specific antibody titers against 5BT was measured by ELISA in serum samples collected at days 0, 13, 27 and 42 post-immunization. Antibody titers were expressed as the reciprocal log10 of the last dilution calculated by interpolation to give an absorbance of 1 above background. Each point corresponds to the geometric mean of each groups. Error bars represent standard error of the mean. Validation of immune response route of recombinant proteins. c 5BT specific total IgG titers detected by ELISA at day 42 post-immunization, d IgG1, and e IgG2a. Endpoint titers were expressed as the reciprocal log10 of serum dilutions. Each symbol represents the value of individual mouse. Horizontal lines indicate the mean of each group of animals