Fig. 4.

Synergistic activity of TtAA9E or TaAA9A in the hydrolysis of two types of pretreated rice straw by cellulase. A solid loading of 5% (w/v) of pretreated rice straw of a ACID or b ALKALI was hydrolyzed using Celluclast 1.5 L (1.4 and 2.8 FPU/g total solids) in the absence or presence of TtAA9E or TaAA9A (5 mg/g cellulose) in 50 mM sodium acetate buffer (pH 5.0) in the presence of 0.02% of NaN₃ and 1 mM of MnSO₄ at 50 °C for 8 days. The reducing sugar was determined by DNS assay, and the reducing sugar yields were expressed as % of the theoretical maximal yield of glucose. The data represent means ± standard errors obtained from the three experimental replicates