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. 2017 Feb 23;17:124. doi: 10.1186/s12906-017-1636-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The growth inhibitory properties of alkaloid extracts from C. zeyheri on Mycobacterium smegmatis. The broth microdilution method was used to determine growth inhibitory properties of extracts using a 96-well microtitre plate. A minimum inhibitory concentration of 125 μg/ml was observed and the optical densities of the wells were read using a microplate reader (Tecan Genios-Pro microplate reader, GrÖdig, Austria) at 590 nm. Values are the mean of ± SD for n = 4.*** P < 0.001 in comparison with the cells exposed to the zero concentration of extract