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. 2017 Feb 22;18:18. doi: 10.1186/s12863-017-0485-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — qRT-PCR validation of the differentially expressed genes analyzed by RNA-seq. qRT-PCR was perform for nine genes that were identified as differentially expressed between the starving apple snails and satiated apple snails. The expression level of each gene was normalized to the level in the satiated snails. The Y axis shows the relative mRNA expression levels. P ^* < 0.05, P ^** < 0.01