Fig. 6.

Effects of dehydration and rehydration on DNA methylation of the RhMKK9 gene. The effects of dehydration and rehydration on cytosine DNA methylation in RhMKK9 gene (a) were determined by McrBC digestion (b) and Alu I-mediated (c) Chop-PCR assays. a Schematic structure of RhMKK9 gene. The primers used for the Chop-PCR assay are indicated as F1 + R1, F2 + R2, F3 + R3, F4 + R4, and F5 + R5. b McrBC digestion assay. Genomic DNA was digested with McrBC for 3 h and amplified by PCR. c Alu I-mediated Chop-PCR assay. Linearised genomic DNA was digested with Alu I for 3 h, respectively, and then was amplified by PCR. The right panel indicates the Alu I site in each tested region. In each assay, undigested genomic DNA was used as a control. Primers were listed in Additional file 1: Table S2. D0 and D16, dehydration for 0 h and 16 h, respectively; R1, rehydration for 1 h. Note, there is no intron in RhMKK9