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. 2017 Jan 15;31(2):172–183. doi: 10.1101/gad.290635.116

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© 2017 Ritschka et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 6. — Induction of senescence in single cells in livers induces an increase in stem cell markers in the surrounding tissue of the liver. Representative histology of liver sections 6 d after delivery of control (Nras^G12V/D38A-IRES-GFP) or mutant (Nras^G12V-IRES-GFP) transposon constructs. (A) H&E staining, immunohistochemistry for GFP and p21, and SA-β-Gal staining. Bars, 100 µm. (B,C) Immunofluorescence for CD44, GFP, and DAPI (B) and Nestin, GFP, and DAPI (C). Bars, 50 µm. All images are representative of at least three biological replicates.