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. 2017 Jan 15;31(2):197–208. doi: 10.1101/gad.292235.116

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© 2017 Dong et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 1. — Genetic and physical interactions of DA1, BB, and DA2. (A,B) The single loss-of-function da1-ko1 allele interacts with bb-eod1-2 and da2-1 to increase petal area. (A) An image of flower heads showing the sizes of petals. Bar, 5 mm. (B) Petal areas. The values given are means (n = 36) ± SE. (*) P < 0.05; (**) P < 0.01 (Student's t-test) compared with wild-type Col-0. (C,D) The single loss-of-function da1-ko1 allele interacts with bb-eod1-2 and da2-1 to increase seed area. (C) Ten seeds aligned to reveal size differences. Bar, 2 mm. (D) Seed areas. The values given are means (n = 50) ± SE. (*) P < 0.05; (**) P < 0.01 (Student's t-test) compared with wild-type Col-0. (E) Dynamic growth measurements of leaf 1 width in Col-0, da1-1, and da1-1 bb-eod1-2. Lines were fitted to data points using the sigmoidal function of Sigmaplot 13. (F) DA1 interacts with BB in vitro. GST-DA1 interacted with HIS-BB. GST-DA1 did not interact with HIS-BBR, an E3 ligase closely related to BB. GST-GUS (β-glucuronidase) was used as a negative control. (G) Myc-tagged DA1 interacted with BB-GFP after transient coexpression in N. benthamiana leaves. BB-GFP and GFP were coexpressed with Myc-DA1 using Agrobacterium-mediated transient expression in N. benthamiana leaves. Expressed proteins were purified using GFP trap and immunoblotted.