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Positive C3 Nephritic Factor analysis. A: 3 parts normal serum, 1 part patient's serum, Mg++ and EDTA (alternative pathway activation possible); B. 3 parts normal serum + 1 part patient's serum + EDTA (alternative pathway activation not possible); C. Contains 3 parts buffer + 1 part patient's serum (control for patient's serum by itself).
