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. 2017 Feb 23;12(2):e0171872. doi: 10.1371/journal.pone.0171872

Table 2. Elution order of all pigments identified in microalgae strains.

Maximum absorbance spectra in the eluent were detailed for porphyrins (P) and carotenoids (Car).

Peak N° Short name Retention time [min] Porphyrin Carotenoid Occurrence in strains
Soret [blue] I [nm] Red II [nm] Soret:Red I [nm] II [nm] III [nm] %III/II
1 P1 6.6 432 622 9.67 Nsp
2 Chl c3 6.7 456 589 8.79 Eh*
3 Car1 6.8 467 At1; At2*; Am1; Am2
4 P2 7.3 446 631 8.33 Cmu; Ccp; Ctl; Oa1*; Ig*; Cg*; Pt; At1; At2
5 P3 7.7 441 630 6.73 Nsp
6 P4 8.1 434 628 5.83 Gs
7 P5 8.1 458 636 9.00 At1
8 P6 8.2 436 651 4.40 Cr
9 P7 8.2 434 629 6.13 Sg
10 Chlide a 8.4 432 666 0.98 Cb
11 P8 8.6 436 656 5.20 Cr
12 P9 8.8 440 631 6.56 Ot
13 Chl c2 8.9 447 633 7.90 Rs; Ctl; Cmu; Oa2; Eh; Tl; At1†; At2; Am2;
14 P10 (Chl c2 like) 8.9 447 634 7.53 Tp; Cg; Ig; Oa1; Cmi; Pt*
15 P11 8.9 445 666 6.01 Ccp
16 P12 8.9 440 666 2.06 Pt
17 P13 8.9 435 666 1.31 So
18 P14 8.9 436 666 1.30 Sg
19 P15 8.9 445 666 3.06 Cc
20 P16 (Chl c1 like) 9.2 444 633 6.48 Tp; Pt†; Cg; Ccp; Ctl; Cmu; Oa1; Oa2; Tl; Ig
21 P17 9.2 443 634 5.53 Sg
22 P18 9.2 445 633 9.2 Cc
23 P19 11.0 432 667 1.00 Pt; Sg
24 Car2 11.9 453 Ig; Tl
25 Pheide a 12.1 409 666 2.24 Gs
26 Peri 12.3 476 At1; At2; Am1; Am2
27 P20 12.8 410 665 1.97 Cr
28 Peri-iso 12.8 477 At1; At2; Am1; Am2
29 P21 13.8 431 665 1.00 Cb; Pt; Sg; Gs; So
30 Car3 14.1 470 Ctl; Nsp
31 Car4 14.1 468 At1
32 PCar1 14.2 422 666 9.12 470 Am2
33 Car5 14.6 449 Oa1
34 Car6 15.0 454 474 218 Ot
35 Car7 15.5 462 At1; At2; Am1; Am2
36 Car 8 15.5 440 467 48 Bn; Ts
37 Car9 15.5 442 468 81 Cr
38 Car 10 15.9 468 At1; At2; Am1; Am2
39 Car 11 16.3 418 441 470 103 Cr
40 Car12 (t-neo-like) 16.3 417 441 470 89 Bn; Ep; Ts; Ot; Cv; Ca; Dsp; Ds; Hp; So; Saa
41 Car13 16.7 484 Am1
42 Fuco 16.9 453 Tp; Pt; Cc; Cg; Ccp; Ctl; Cmi; Cmu; Nsp; Oa1; Oa2; Sg; Eh; Tl; Ig
43 Car14 17.5 479 At2; Am1; Am2
44 Car15 (Loro like) 17.5 445 472 41 Bn; Cr; Ts; So; Saa
45 9-cis Neo 17.6 413 436 465 86 Bn; Ep; Cr; Ts; Ot; Cv; Ca; Dsp; Ds; Hp; Cb; So; Saa
46 Car16 17.7 475 At1*; At2; Am1; Am2
47 Car17 17.9 425 447 476 62 At1; At2; Am1; Am2
48 19-hex k Fuco 17.9 448 469 85 Eh
49 Pras 18.4 455 Ot
50 Viola 18.8 416 439 469 89 Bn; Cr; Ig; Ts; Ot; Cv; Ca; Dsp; Ds; Hp; Cb; So; Saa
51 Asta 19.4 479 Cr; Saa
52 Car18 19.8 459 Ot
53 19’-Hex-Fuco 19.2 447 462 468 9 Eh
54 Car19 (cis-Fuco like) 20.2 443 Tp; Pt; Cc; Cg; Ccp; Ctl; Cmi; Cmu; Nsp; Sg; Tl; Ig; Oa1; Oa2
55 Car20 20.4 403 428 455 64 Am1; Am2; At1; At2
56 Diadino 20.6 422 446 475 54 Ep; Tp†; Pt; Cc; Cg; Ccp; Ctl; Cmi†; Cmu; Nsp; Oa1*; Oa2; Sg; Eh; Tl; Ig; Am1; Am2; At1; At2
57 Car21 20.8 411 434 462 68 Bn; Ts; Dsp*
58 Car22 20.9 458 Ep
59 Car23 21.5 440 466 55 Cr
60 Car24 21.5 442 467 42 Ts
61 Car25 21.5 443 469 22 Ot; Ca; Ds
62 Car26 21.5 415 437 464 82 Cv
63 Car27 21.5 417 438 465 70 Saa; Hp
64 Anthe 21.7 445 473 60 Dsp; Bn; Cb
65 Car28 21.9 419 439 468 37 Ep
66 Car29 22.1 471 Cr
67 Allo 22.1 452 479 32 Rs; Am1
68 Car30 22.4 421 441 470 67 Ep
69 Car31 22.4 452 At2
70 Car32 22.6 420 441 470 58 Ep
71 Car33 23.1 445 462 475 58 Rs
72 Diato 23.2 469 478 32 Tp; Cc; Cg; Ccp; Ctl; Cmi; Cmu*; Nsp; Oa1; Oa2; Sg; Eh; Tl; Ig; Ep*; Am1; Am2*; At1
73 P22 23.6 429 659 1.00 Gs
74 Zea 23.9 451 477 25 Bn; Cr; Cb; Cp; Ts*; Ot; Cv; Ca; Dsp; Ds; Hp; So; Saa; Rv; Pp; Gs; Cg*
75 Lut 24.2 445 472 60 Bn; Cr; Ts; Cv; Ca; Ds; Dsp; Hp; Cb; So; Saa
76 Car34 24.6 427 453 59 Ot
77 P23 24.8 438 688 5.01 Ts
78 Car35 24.8 420 438 465 41 Dsp; Ds; Hp; Cb; So; Saa; Cv
79 PCar 2 24.8 438 688 19.3 416 438 465 9 Ca
80 Car36 25.0 444 469 10 Cp; Gs; Rv; Pp*
81 Cantha 25.5 476 No presence in analyzed strains
82 Car37 25.8 419 439 467 61 Ts*; Hp*; So; Saa
83 Car38 25.9 419 440 467 59 Cv; Ca; Ds; Dsp;
84 Car39 26.0 445 471 31 Gs; Rv; Pp
85 Car40 26.5 461 Ep
86 Car41 27.6 446 473 37 Ts; Bn
87 P24 27.6 420 655 1.84 Cg*; Cmu; Nsp
88 P25 27.6 420 655 1.88 Ccp; Ctl
89 P26 27.7 456 635 1.84 Hp; Saa
90 P27 28.6 436 661 1.23 Bn
91 Car42 29.0 446 473 38 Bn; Cr; Ts
92 Chl b 29.7 468 651 2.80 Bn; Ep; Cr; Ts; Ot; Cv; Ca; Dsp; Ds; Hp; So; Saa; Cb
93 Car43 29.9 468 Tl
94 Car44 (Croco like) 30.2 446 472 50 Rs
95 Chl b-epi 30.3 468 651 2.80 Bn; Ep; Ts; Ot; Cv; Ca; Dsp; Ds; So; Hp
96 Car45 31.0 445 471 25 Rv
97 P28 (chl a allomer) 31.1 420 655 1.78 Tp; Pt; Cc; Cg; Ccp; Ctl; Cmi; Cmu; Nsp; Oa1; Hp; Saa; At1; At2
98 Car46 31.2 446 472 50 Bn
99 Car47 (Cryp) 31.2 451 477 24 Gs; Rv; Pp
100 P29 (chl a allomer) 31.3 419 662 1.63 Pt*; Cc*; Ccp; Ot
101 P30 (chl a allomer) 31.5 430 664 1.11 Ts
102 P31 (chl a allomer) 31.5 432 665 1.10 Cv
103 P32 (chl a allomer) 31.5 422 664 1.20 Pt; Cc*; Ccp; Ctl; Ot
104 P33 (chl a allomer) 31.5 429 665 1.11 Nsp*; Oa1*; Cmi; Cmu*; Ca; Hp*; Saa; At1; At2
105 Car48 31.8 474 Tl
106 Chl a 32.3 431 665 1.00 All strains
107 P34 32.6 422 666 3.53 Nsp; Ccp
108 Chl a -epi 32.8 431 665 1.00 All strains with At1*; At2*; [excepted Eh]
109 Car49 33.1 472 Ep
110 Car50 33.4 425 451 44 Gs
111 P35 33.7 438 655 4.95 Cr
112 PCar3 34.5 445 666 6.67 416 445 474 56 At1
113 PCar4 34.5 415 666 5.16 415 444 474 37 Am1
114 PCar5 34.5 413 666 3.95 412 445 473 20 Am2
115 Car51 34.6 442 469 55 Ot
116 Car52 35.0 432 453 482 31 Dsp
117 PCar6 35.2 468 653 8.66 441 468 498 9 Ts
118 Car53 35.6 416 439 465 33 Cv
119 Car54 35.7 438 459 488 47 Ts; Ds; Dsp
120 Car55 35.8 444 467 9 Bn; Pt*; Cmu; Cb*; Cp; Gs; Ot; Cv; Ca*; Rv; Pp; Hp; Sg*; Ccp*; Ctl*; Nsp*; Oa1*; Oa2*
121 βε-Car 36.4 444 472 56 Rs; Ts; Cv; So; Cb; Cr
122 ββ-Car 36.5 450 475 15 All strains
123 Car56 37.1 440 466 28 Cp; Dsp; So; Cb; Ts; Bn
124 Car57 37.1 442 466 11 Gs

Pigment standards are written in italic characters. Pigment characteristics of one strain or several strains within a particular species are written in bold characters. Strains marked with an asterisk cross (†) indicates that the pigment is abundant and fully characterized in literature. Strains marked with an asterisk (*) have a match factor of 999 in visible spectra.