Fig 2. Curcumin inhibits 95D cell migration and invasion.

(A) 95D confluent monolayer cells are scratched with a pipette tip and then treated with 10 μM or 20 μM curcumin. Representative images show the inhibitory effect of curcumin on 95D cell at 24 h. (B) The gap distance is quantitatively evaluated using the Image J software. (C) The invasion ability of 95D cell is determined by invasion assay. Cells in low surface of the Boyden chamber are stained and photographed under a light microscope at × 100 magnification. (D) The invading cells are quantified by counting the number of stained cells under a light microscope at × 200 magnification. Columns, mean from three different experiments with 3 duplicates; *, p < 0.05; **, p < 0.01 compared with 0 μM curcumin group.