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. 2017 Feb 23;12(2):e0171256. doi: 10.1371/journal.pone.0171256

Fig 3. Riluzole induces apoptosis in LM7 and OS482 cells.

Fig 3

LM7 cells were seeded and treated with Riluzole at various concentrations or DMSO for control for 72 hours. The cells were fixed and the TUNEL assay was performed. Images were captured and analyzed. A. Blue bars represent DAPI positive cells and red bars represent TUNEL positive cells. B. Percentage of TUNEL positive cells in each sample was calculated based in total number of DAPI positive cells for that sample (P value <0.001 = ***). C. Representative images of the TUNEL assay. D. OS482 were treated with various concentrations of Riluzole or DMSO for control and TUNEL assay was performed as in A. E Percentage of TUNEL positive cells in each sample was calculated for OS482 cells based in total number of DAPI positive cells for that sample (P value <0.001 = ***).