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. 2016 Nov 10;18(1):16–25. doi: 10.1080/15384047.2016.1252880

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© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Cisplatin downregulates Par-4 in a dose- and proteasome-dependent manner. (a) Western blots showing Par-4 expression levels in TRIM21-transfected HCT-116 cells over time at different doses of cisplatin. Cisplatin doses are in units of μg/ml. Time is in units of hours. Actin is shown as a loading control. (b) HCT-116 cells were transfected with or without TRIM21 expression plasmid for 48 hrs, then treated with the indicated doses of cisplatin in μg/ml for 24 hrs, and with or without 10 μM MG132. Blot shows Par-4 expression levels, and actin is shown as a loading control.