Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Cisplatin downregulates Par-4 in both the cytoplasmic and nuclear compartments. HCT-116 cells were either transfected with plasmid encoding TRIM21 or control plasmid for 48 hrs and treated with or without 3.75 μg/ml of cisplatin. Then, nuclear-cytoplasmic fractionation was performed and Par-4 and TRIM21 levels were examined by Western blotting, with Lamin A/B and β-tubulin serving as markers validating the integrity of the fractionation.
