Table 1.

HCT-116, HT-29, and KM12C cells were grown and either transfected with Par-4 expression plasmid or control plasmid for 48 hrs. Par-4 was immunoprecipitated from whole cell lysates using anti-Par-4 antibody and protein G magnetic beads. An in-solution trypsin digestion was performed on the eluted proteins, and the digests were sent for mass spectrometry analysis. A representative list of the most abundant proteins identified in the digest reveals TRIM21 as a potential novel interacting partner of Par-4.

IP α HA		IP α Par-4
Accession	Name	Accesaon	Name
gi | 62414289	vimentin [Homo sapiens]	gi | 55769533	PRKC apoptosis WT1 regulator protein [Homo sapiens]
gi | 58331171	T-complex protein 1 subunit zeta isoform b [Homo sapiens]	gi | 15208660	E3 ubiquitin-protein ligase TRIM21 [Homo sapiens]
gi | 4502643	T-complex protein 1 subunit zeta isoform a [Homo sapiens]	gi | 4826998	splicing factor, proline- and glutamine-rich [Homo sapiens]
gi | 31542947	60 kDa heat shock protein, mitochondrial [Homo sapiens]	cont | 000394 | Trypa5	cont | 000394 | Trypa5|P romTArt5 Promega trypsin artifact 5 К to R mods (22391, 2914)(1987, 2003).
RRRRRgi | 62422577	REVERSED neurobeachin isoform 1 [Homo sapiens]	gi | 4506583	replication protein A 70 kDa DNA-binding subunit [Homo sapiens]