Figure 8.

Schedule of CIK cells differentiation and a HCC mouse model generation. Schedule of CIK cells differentiation and a HCC mouse model generation. MH-134 cells were cultured for 7 d. 4 d before MH-134 cell injection to C3H mice, splenocytes were obtained by sacrificing other 5-week-old C3H mice. With these splenocytes, CIK cells were cultured for 14 d. Interventions (control, VPA, CIK cells, CIK cells + VPA) were performed at D0. After then, each tumor volume was observed for 7 d. All mice were killed at D7 to acquire their tumors for analysis.