Figure 2.

Metuzumab combined with GP represses cell proliferation and induces apoptosis in vivo. (A) Western blot analysis on the level of CD147 expression in A549, NCI-H460 or NCI-H520 cells. β-actin was used as the internal control. The experiments were performed triplicate. Gray-scale value was analyzed by Image J software for 3 times. The numbers indicate relative protein expression normalized to β-actin. (B) FACS analysis was used to examine the level of CD147 expression in A549, NCI-H460 or NCI-H520 cells. (C) Representative images of immunohistochemical staining for Ki-67, TUNEL, Bax and Bcl-2 in nude mice bearing A549 cell xenograft received metuzumab, GP or metuzumab combined with GP treatment. Scale bar in Ki-67, Bax and Bcl-2 represents 50 μm, scale bar in TUNEL represents 20μm. Histogram represents the integrated optical density (IOD) of Ki-67, Bax and Bcl-2 expression and percentage of TUNEL positive nuclei from the studied group. The bars represent each sample performed in triplicate, and the error bars indicate mean ± SD. **p < 0.01. ***p < 0.001.