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. 2017 Feb 8;13(2):e1006600. doi: 10.1371/journal.pgen.1006600

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© 2017 Joshi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A. Gene expression was quantified by RT-qPCR during PM differentiation after transfection with the indicated siRNAs. B. Fluorescence microscopy images after 6 days of differentiation of PMs transfected with the indicated siRNAs. Green channel shows staining with Myh antibody, and blue Dapi-stained nuclei. Scale bar 100 μm. C. Fusion index of siControl and siTead-transfected cells. D. Quantification of gene expression after transfection with the indicated siRNAs. In panels data was analysed by multiple t-tests. P-values as above. Fusion index was generated from counting around 500 nuclei in each condition from 3 biological replicates.