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. 2016 Apr 28;16(3):251–258. doi: 10.1080/15384101.2016.1175798

Figure 2.

Figure 2.

Gamma H2AX staining. Fluorescence photomicrographs show the merging of cells stained with anti-H2AX (green), anti Ki-67 (red) and DAPI (blue). Representative microscopic fields are shown. The graph shows the degree of H2AX phosphorylation. This was evaluated by counting the number of gamma-H2AX immunofluorescent foci per cell. Foci number was determined for 200 cells. Each dot represents an individual cell. Horizontal bars indicate mean value for each category. shR1, shR2 and sh107 cells were tested vs. control MSCs (shSCR, #p < 0.05). In each silenced condition (shSCR, shR1, shR2 and sh107), we compared irradiated versus unirradiated cells (*p < 0.05; **p < 0.01). The shSCR wild type MSCs; shR1, shR2 and sh107 are MSCs with silenced RB1, RB2/P130 and P107, respectively.