Figure 10. fALOD4 binding to RBCs of patients on hemodialysis.
(A) Blood samples were collected from 50 hemodialysis patients immediately before dialysis was initiated and from 44 ethnicity-matched individuals who did not have chronic renal failure and were measured on the same day (Table 6). RBCs were isolated and fALOD4 binding assays were performed as described in the Materials and methods. Each measurement was performed in triplicate. fALOD4 binding values were normalized to the binding values obtained for the reference blood sample that was collected from the same healthy subject for each experiment. Data is plotted as the mean of each nomalized value. *p<0.0001, two-tailed t-test. (B) fALOD4 binding measurements were made exactly as described above using RBCs isolated from blood samples collected before and after dialysis in eight individuals undergoing hemodialysis. Blood samples were collected once from each patient before and after hemodialysis in eight patients. Data is plotted as the mean ± SEM.