Figure 3. Characterization of RBC cholesterol accessibility assay.
(A) Intra-assay correlation was determined by measuring fALOD4 binding to two aliquots of RBCs from 164 unrelated healthy individuals as described in the Materials and methods. The levels of fALOD4 binding were normalized to a reference sample of RBCs from a healthy individual that was included in all the assays. Data points represent the mean of three independent measurements from each of the RBC aliquots. (B) Intra-individual variability of fALOD4 binding to RBCs from five individuals. Blood samples from five unrelated individuals were collected on three separate days over a month and fALOD4 binding was measured. fALOD4 binding values in A and B were normalized to the binding values obtained from the reference blood sample. The reference blood sample was collected from the same healthy volunteer prior to each experiment in this study and the sample was processed and assayed concurrently. Error bars represent the mean ± SEM of three measurements from each blood sample.