Figure 9. Effect of phospholipase and sphingomyelinase treatment of RBCs on fALOD4 binding.
A total of 1.6 × 108 RBCs were incubated with indicated the amounts of active or inactivated phospholipase A2 (PLA2) (A) or active or inactive sphingomyelinase (SMase) (B) for 1 hr at room temperature or 37°C, respectively. Cells were washed three times in PBS and an aliquot containing 2 × 105 RBCs was used to measure fALOD4 binding. Data points represent the mean of three independent measurements (±SEM). The experiment was repeated three times and the results were similar.