Figure 2. Impaired spatial learning and memory in Nestin-Cre; Snx6^fl/fl mice.

(A–I) No effects of SNX6 ablation on the performance in assays of rotarod (A) (13 Snx6^fl/fl and 16 Nestin-Cre; Snx6^fl/fl mice), open field (B) (23 Snx6^fl/fl and 23 Nestin-Cre; Snx6^fl/fl mice), elevated plus maze (C) (14 Snx6^fl/fl and 13 Nestin-Cre; Snx6^fl/fl mice), tail suspension (D) (14 Snx6^fl/fl and 24 Nestin-Cre; Snx6^fl/fl mice), forced swimming (E) (15 Snx6^fl/fl and 25 Nestin-Cre; Snx6^fl/fl mice), Three-Chamber test (F) (10 Snx6^fl/fl and 9 Nestin-Cre; Snx6^fl/fl mice), repetitive behaviors (G) (12 Snx6^fl/fl and 10 Nestin-Cre; Snx6^fl/fl mice), Y maze (H) (11 Snx6^fl/fl and 15 Nestin-Cre; Snx6^fl/fl mice) and shuttle box (I) (20 Snx6^fl/fl and 13 Nestin-Cre; Snx6^fl/fl mice). The data represent mean ± SEM for each group. (J–K) Increased escape latency at acquisition learning (J) (data represent mean ± SEM of four trials per day), decreased number of crossing and increased latency to first enter the 1.5x area at probe test (K) (the data represent mean ± SEM for each group) in Nestin-Cre; Snx6^fl/fl mice in the Morris water maze. Subject numbers were 18 Snx6^fl/fl and 22 Nestin-Cre; Snx6^fl/fl mice. (L) After a 20-day rest, both Snx6^fl/fl and Nestin-Cre; Snx6^fl/fl mice exhibited memory extinguishment. (M) Decreased number of crossing and increased latency to first enter the 1.5x area at probe test in Nestin-Cre; Snx6^fl/fl mice after one recall training. The data represent mean ± SEM. N = 3 independent experiments.

DOI: http://dx.doi.org/10.7554/eLife.20991.005