Video 7. Time-lapse live imaging showing movement of EEA1- and Homer1c-labeled vesicles in dendrite.
Download video file (1.5MB, mp4)
DOI: 10.7554/eLife.20991.022
Hippocampal neurons co-transfected with EEA1-YFP and mCherry-Homer1c expressing constructs were imaged live by TIR-FM. Yellow arrowheads indicate the trajectory of an EEA1-, Homer1c-positive vesicle detaching and moving away from a large structure, suggesting fission and formation of transport carriers from early endosomes. White arrowheads indicate the trajectory of another vesicle moving in the dendritic shaft. Images were acquired at 2 frames/s. Video plays at 50 frames/s. Bar: 1 μm.