Figure 4. STG neurons present variable branch point numbers and angles.
Branch points numbers and angles were extracted from three-dimensional skeletal reconstructions generated in KNOSSOS from high-resolution confocal image stacks of Lucifer yellow neuronal dye-fills. (A) Branch point numbers were highly variable across neuron types and GM neurons presented higher branch point numbers than other cell types (letters are indicative of ANOVA results; [F(3,12)=7.12, p = 0.0053]; Tukey HSD). (B) Angles were calculated at bifurcating branch points as the angle (purple) between a given daughter branch (D1 or D2) and the hypothetical continuation of the parent (P) branch (dashed line). (C) Branch angle histograms show a wide range of branch point geometries within single neurons. Sets of four concentric histograms show the branch angle distributions of four neurons of a given neuron type (indicated by color in key on right). Solid lines indicate means, dashed lines indicate medians. Shaded ranges of histograms are indicative of the 25–75% confidence interval. There were no measurable statistical differences across individual neurons or neuron types).