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. Author manuscript; available in PMC: 2017 Feb 23.
Published in final edited form as: Cell Rep. 2016 Jul 21;16(5):1379–1390. doi: 10.1016/j.celrep.2016.06.095

Figure 3. Auf1−/− satellite cells are unable to self-renew once activated.

Figure 3

(A) Immunofluorescence analysis for expression of laminin (AF-488, green), PAX7 expression (AF555, red), and nuclei (DAPI, blue) in 7 or 15 day post-injury skeletal muscle in 4 month old WT and KO mice. TA muscle was injured by BaCl2 injection. TA muscles were frozen in OCT, 5 images from 3 sections were analyzed per mouse (scale bar 50 μm). DAPI + 2nd is a control, sections stained with DAPI and secondary antibody only.

(B) Quantification of fiber size by Minimum Feret’s Diameter determined by ImageJ64 at 15 day post-injury in WT (black) and KO (grey) mice. Bars denoting significance represent the range in which the Minimum Feret’s Diameter is significantly different between WT and auf1 KO TA muscle. *P<0.05, **P<0.005, unpaired t-test.

(C) Quantification of PAX7 expression in WT and KO mice in uninjured (black), 7 day post-injury (patterned), and 15 day post-injury (grey) skeletal muscle. *P<0.05, **P<0.005, 2way ANOVA.