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. 2016 Apr 8;7(40):64766–64777. doi: 10.18632/oncotarget.8649

Figure 3. Knockdown of COX-2 partially resensitizes DiFi5 cells to cetuximab via induction of apoptosis.

Figure 3

DiFi, DiFi5, and DiFi5 COX-2 knockdown clones were treated with 20 nM cetuximab or not for 24 h. Cell samples were subjected to flow cytometry analysis after staining with FITC-labeled Annexin V. Top: Individual comparisons for the sum of Q2 (late apoptotic cells) and Q3 (early apoptotic cells) derived from the contour plots shown at the bottom. KD, knockdown. Unpaired Student t test was performed. *p < 0.05; ns, not significant. Bottom: FITC-Annexin V versus propidium iodide contour plots with quadrant gates showing four populations of DiFi, DiFi5, and DiFi5 COX-2 knockdown clones with and without cetuximab treatment.