Table 1. Immunohistochemical expression characteristics of TP53 mutations.
| Mutation features | p53 immunohistochemistry | p-valuea | ||||
|---|---|---|---|---|---|---|
| Total (n = 7878) | Negative (%) (n = 1852) | Positive (%) (n = 6026) | ||||
| Exons/introns | ||||||
| exons | 7690 | 1745 | (22.69) | 5945 | (77.31) | 0.001 |
| introns | 186 | 105 | (56.45) | 2086 | (43.55) | |
| NA | 2 | 2 | (100.00) | 0 | (0.00) | |
| CpG site | ||||||
| no | 5373 | 1433 | (26.67) | 3940 | (73.33) | < 0.0001 |
| yes | 2505 | 419 | (16.73) | 2086 | (83.27) | < 0.0001 |
| Splice site | ||||||
| alternative | 71 | 12 | (16.90) | 59 | (83.10) | 0.1872 |
| consensus | 112 | 72 | (64.29) | 40 | (35.71) | < 0.0001 |
| criptic | 62 | 16 | (25.81) | 46 | (74.19) | 0.6684 |
| no | 7633 | 1752 | (22.95) | 5881 | (77.05) | < 0.0001 |
| Type | ||||||
| Single nucleotide mutationsb | 6860 | 1324 | (19.30) | 5536 | (80.70) | <0.0001 |
| tandem | 97 | 6 | (6.19) | 91 | (93.81) | 5.16E-05 |
| complex | 48 | 18 | (37.50) | 30 | (72.50) | 0.0219 |
| deletion | 667 | 380 | (56.97) | 287 | (43.03) | <0.0001 |
| insertion | 196 | 117 | (59.69) | 79 | (40.31) | <0.0001 |
| NA | 10 | 7 | (70.00) | 3 | (30.00) | 0.000522 |
| Effects | ||||||
| frameshift | 677 | 451 | (66.62) | 226 | (33.38) | <0.0001 |
| intronic | 53 | 25 | (47.17) | 28 | (52.83) | 4.58E-05 |
| large deletion | 2 | 2 | (100.00) | 0 | (0.00) | 0.0107 |
| missense | 5889 | 707 | (12.01) | 5182 | (87.99) | <0.0001 |
| nonsense | 552 | 393 | (71.20) | 159 | (28.80) | <0.0001 |
| silent | 367 | 136 | (37.06) | 231 | (62.94) | 3.64E-10 |
| splice | 131 | 86 | (65.65) | 45 | (34.35) | <0.0001 |
| other | 177 | 44 | (24.86) | 133 | (75.14) | 0.6683 |
| NA | 30 | 8 | (26.67) | 22 | (73.33) | 0.6828 |
| Structural motif | ||||||
| C-terminal | 234 | 104 | (44.44) | 130 | (55.56) | <0.0001 |
| C- terminal/NLS | 31 | 13 | (41.94) | 18 | (58.06) | 0.0153 |
| C- terminal/tetramerization | 101 | 39 | (38.61) | 62 | (61.39) | 3.15E-04 |
| L1/S/H2 | 1693 | 234 | (13.82) | 1459 | (86.18) | <0.0001 |
| L2/L3 | 2765 | 434 | (15.70) | 2331 | (84.30) | <0.0001 |
| N- terminal | 34 | 26 | (76.47) | 8 | (23.53) | <0.0001 |
| N- terminal/Transactivation | 33 | 20 | (60.61) | 13 | (39.39) | <0.0001 |
| N- terminal/Transactivation/ NES | 5 | 2 | (40.00) | 3 | (60.00) | 0.3844 |
| NDBL/β-sheets | 2657 | 795 | (29.92) | 1862 | (70.08) | <0.0001 |
| SH3-like/Proline-rich | 101 | 60 | (59.41) | 41 | (40.59) | <0.0001 |
| NA | 224 | 125 | (55.80) | 99 | (44.20) | <0.0001 |
a
Comparisons between groups were performed with Pearson's Chi-square (χ2), Significant p-values are marked in bold;
b
Containing the following point mutations: A:T>C:G, A:T>G:C, A:T>T:A, G:C>A:T, G:C>A:T at CpG, G:C>C:G and G:C>T:A)