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. 2016 Jul 21;7(40):64932–64941. doi: 10.18632/oncotarget.10751

Figure 1. miR-29a/b/c target Tet1 via direct binding to 3′UTR in vitro.

Figure 1

(A) Screen the miRNAs which are predicted to target Tet1 with PicTar software via dual luciferase reporter assay. A 1698bp mouse Tet1 3′UTR was cloned into a luciferase reporter vector, and cotransfected with different miRNA mimics to 293T cells. Luciferase activity was measured 48 h after transfection. Data are shown as mean ± SEM (n = 3). (B) miR-29a inhibitor increased the relative luciferase activity of vectors containing Tet1 3′UTR. Data are shown as mean ± SEM (n = 6). (C) 8 putative miR-29a/b/c target sites are present in Tet1 3′UTR. (D) miR-29a/b/c cannot inhibit the relative luciferase activity of vectors containing Tet1 3′UTR with all 8 putative binding sites mutated. Data are shown as mean ± SEM (n = 3). ns means no significance, *p < 0.05, **p < 0.01, ***p < 0.001.