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. 2016 Aug 18;7(40):65109–65124. doi: 10.18632/oncotarget.11371

Figure 2. PPARγ modulates the transcriptional activity of CXCR4 gene promoter containing a putative PPAR response element (PPRE).

Figure 2

(A) Schematic representation of the CXCR4 promoter constructs used in this study (left panel). MCF-7 cells were transiently transfected with luciferase plasmids containing the CXCR4 promoter (p-2300) and its deleted constructs (p-2144 and p-1507) and then treated with vehicle (−), BRL 10 μM with or without GW 10 μM for 12 h (right panel). The results are expressed as fold change respect to the vehicle-treated cells (−). The results are mean ± SD of three different experiments, each performed with triplicate samples. *P < 0.05. n.s. = not significant. (B) Chromosomal localization of the human cxcr4 gene at chromosome 2 (left panel). A shot from NCBI genome browser to illustrate the localization of cxcr4 gene. The location of Peroxisome proliferator response element (PPRE)-like is highlighted by vertical line and zoomed-in to view the genomic sequence spanning from 136119907 to 136119895 base pair in the negative strand (right panel) (C) The genomic sequence of the PPRE-like motif within CXCR4 promoter is aligned to a logo graphic representation of PPRE sequence generated using a PPRE collection with WebLogo [48].