Skip to main content
. 2016 Aug 18;7(40):65109–65124. doi: 10.18632/oncotarget.11371

Figure 4. Effects of BRL on motility and invasion of MCF-7 and MDA-MB-231 breast cancer cells.

Figure 4

Wound-healing (A), transmigration (B) and invasion (C) assays in breast cancer cells treated with vehicle (−), BRL 10 μM with or without GW 10 μM for 24 h. Small squares: time 0. Histograms in A represent the mean ± SD of three separate experiments in which migrated cells were calculated by image analysis using Image J software and expressed as fold change compared to vehicle-treated cells. Migration and invasion were quantified by viewing five-separate fields/membrane (10×-magnification) and expressed as mean numbers of migrated cells. Data represent the mean ± SD of three-independent experiments, assayed in triplicate. *P < 0.05. (D) Immunoblots of phosphorylated levels (p) of FAK, AKT and ERK1/2 and total proteins from cells treated with vehicle (−), BRL 10 μM with or without GW 10 μM for 24 h. Numbers below the blots represent the average fold change between phosphorylated and total protein and GAPDH protein expression vs vehicle-treated cells. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.