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FCM of annxin V-FITC/PI dual staining was performed and cleaved-caspase 3 protein expression level was detected to evaluate apoptosis in cells treated with NaF alone, pre-incubated with SRT1720 or pre-incubated with Ex-527. (A) The apoptotic rates were detected by FCM of annxin V-FITC/PI dual staining. (B) The cleaved-caspase 3 expression levels were detected using Western blot assay. (C) Results of densitometric analysis for Western blot.
