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. 2016 Aug 31;7(40):65418–65428. doi: 10.18632/oncotarget.11739

Figure 5. Interference of expression of HA receptors does not affect the inhibition of CHAG on cancer cells.

Figure 5

(A and B) The influence of expression interference of CD44 on the inhibitory effect of CHAG. AGS and HepG2 cells were transfected with CD44 siRNA or control siRNA (negative control, NC) for 36 h. And then in the EGF group, the cells were treated with EGF (100 ng/ml) for 5 min. In the CHAG + EGF and CD44 siRNA + CHAG + EGF groups, the cells were treated with two concentrations of CHAG (500, 1000 μg/ml) for 1 h and then with EGF (100 ng/ml) for 5 min. The cells were harvested and the lysates were subjected to Western blotting with corresponding antibodies. (C and D) The influence of expression interference of RHAMM on the inhibitory effect of CHAG. The cells were transfected with RHAMM siRNA or control siRNA (negative control, NC) for 36 h, and following treatments were same to panel A and B. The results were representatives of three independent experiments. (E and F): The results of densitometry analysis of Western blotting results of the corresponding groups. (#P < 0.05, compared with control group; *P < 0.05, **P < 0.01, compared with the EGF group; ΔP < 0.01, compared with the EGF group and P > 0.05, compared with 500 μg/ml CHAG + EGF group; & P < 0.01, compared with the EGF group and P > 0.05, compared with 1000 μg/ml CHAG + EGF group).