Figure 5. TGFβ-mediated PAR-1 upregulation is blocked by Sp1 inhibitors.

A549 lung adenocarcinoma cells were incubated with or without TGFβ (1 ng/ml) for 24 hours and in the presence of the Sp1 inhibitors. Panel A. Mithramycin A (10 μM) for 8, 16 and 24 hours, Panel B. WP631 for 16 hours at the concentration 150 nM and 300 nM. PAR-1 expression was quantified by real-time qPCR. Each data point represents the mean +/− SEM of 3 replicate wells, analysed by Two-way ANOVA, **p<0.01, ***p<0.001 in comparison to vehicle control.